HPLC determination of chronic bronchitis purple pills Bergenin content.docVIP

 PAGE \* MERGEFORMAT 13 HPLC determination of chronic bronchitis purple pills Bergenin content Keywords: HPLC;,, chronic bronchitis purple pill;,, Bergenin Abstract: Objective To establish a chronic bronchitis purple pills Bergenin HPLC (HPLC) determination method. Method uses methanol solution heat reflux extraction Bergenin, using RP18 C18 column as stationary phase, methanol water (25:75) as mobile phase, flow rate 1 ml / min, detection wavelength 275 nm. Results 0.053 5 ~ 0.535 μg range Bergenin with the peak area showed good linear relationship, r = 0.999 9, the average recovery was 98.2%, RSD = 2.15%, n = 9. Conclusion The method is simple, rapid, sensitive, specific and well, is an effective method of quality control preparation. Keywords: HPLC; chronic bronchitis purple pill; Bergenin Determination of Bergenin in Manzhizihong Pills by HPLC Abstract: ObjectiveAn HPLC method for determination of bergenin in Manzhizihong Pills was established.MethodsBergenin was extracted by methanol and warmup and reflux. An RP C18 column was used with the mobile phase of methanol-water (25:75), at the detection wavelength of 275 nm and the flow rate was 1.0ml/min. ResultsThe linear range was 0.0535 ~ 0.535 and the average recovery was 98.2% (RSD 2.15%). ConclusionThe method is rapid, convenient. Keywords:: HPLC; Manzhizihong Pills; Bergenin Chronic bronchitis purple pills by the dwarf in tea, red back leaf root, pig powder, composed of five fingers peaches and yams, with spleen dewetting, Shun gas phlegm, anti-inflammatory efficacy of antitussive mainly used for cough, phlegm, gas promotion, chronic bronchitis and other diseases [1]. In this paper, HPLC method for the drug in Bergenin had determination. Precision, repeatability were good and reliable results can be used as drug quality standards of the quantitative methods [2,3]. An instrument and reagent 1.1 Instrument Waters600 liquid chromatography (Waters