HPLC determination of ginsenosides Rg1 Danqi the content of the film.docVIP

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HPLC determination of ginsenosides Rg1 Danqi the content of the film.doc

HPLC determination of ginsenosides Rg1 Danqi the content of the film

 PAGE \* MERGEFORMAT 6 HPLC determination of ginsenosides Rg1 Danqi the content of the film [Abstract] Objective] Dan determination of seven methods. [Method] HPLC, mobile phase: methanol: water (48:52, column: DiamonsiLTM C18 (250mm 4.6mm, 5um), detection wavelength: 280nm . [Results] ginsenoside Rg1 in the linear range of 4.8g ~ 24.0g r = 0.9996, average recovery was 99.80%, RSD 1.46%. [Conclusion] The method can be used to control the quality of Dan seven. [Keywords:] HPLC, Dan seven, ginsenoside, determination [Objective] To establish a HPLC method or the determination of ginsenside Rg1 in Danqi tablet. [Method] HPLC conditions were as fellows: DiamonsiLTM C18 colum, a mobile phase of MeOH: H2O (48:52), and the wavelength at 280nm. [Results] There was a good linearity within the rang of 4.8ug ~ 24.0ug of ginsenside Rg1.The average recover was 99.80% and RSD was 1.46%. [Conclusion] This method can be used for the quality control of Danqi tablet.Keywords:: HPLC, Danqi tablet, ginsenside Rg1, determination Dan seven by the SM, Panax is made of two herbs, with the efficacy of promoting blood circulation, are widely used in blood stasis and qi stagnation, heart Bitongkang embolism. The current standards set out in the Ministry of Health received the standard Chinese medicines pharmaceutical preparations first books, but only microscopic, and physical and chemical identification [1], to improve the detection means to more effectively control the product’s inherent quality, this paper, HPLC method was determination of Panax. experiment with satisfactory results. An instrument and reagent waters high performance liquid chromatography. HS chromatography data station (Spectra Technology Development Co., Ltd. Hangzhou Britain. ginsenoside Rg1 reference substance (China Pharmaceutical and Biological Products, lot number 110703200323, Dan seven (Guangzhou Baiyun Mountain pharmaceutical 050,125 050 713 051 223, the reagents used were of analytical grad

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