HPLC determination of ferulic acid in scorpion capsule content.docVIP

 PAGE \* MERGEFORMAT 6 HPLC determination of ferulic acid in scorpion capsule content Author: Lijin Shen Liang Chen Fang Chun-Xu Yang [Abstract] Objective To determine the scorpion capsule of ferulic acid in high-performance liquid chromatography. Methods VP-ODS (250 mm * 4.6 mm, 5 μ m) column, acetonitrile -1% acetic acid solution (25:75) as mobile phase, flow rate of 1 ml / min, detection wavelength: 313 nm. The results of ferulic acid in the 2.5 ~ 25 μ g / ml concentration range, the peak area and concentration a good linear relationship, correlation coefficient r = 0.999 8, plus sample average recovery was 99.43%, RSD = 2.15% (n = 6). Conclusion The method is simple, accurate, and can be used for scorpion capsule in the determination of ferulic acid. [Keywords:] scorpion capsule; ferulic acid; high-performance liquid chromatography Abstract: ObjectiveTo establish an HPLC method for determination of Ferulic acid content in Xiebao capsule.MethodsSamples were determined by RP-HPLC on a VP-ODS column (250 mm * 4.6 mm, 5 μ m) with a mixture of acetonitrile-1% acetic acid (25:75) as mobile phase at the flow velocity of 1 ml / min and UV detection at 320nm. ResultsThe calibration curves of Ferulic acid was linear from 2.5 to 25μ g/ml (r = 0.999 8, n = 6), the average recovery and RSD were 99.43% and 2.15% (n = 6) respectively. ConclusionThe method is simple and accurate. It can be used for determination of Ferulic acid content in Xiebao capsule. Keywords:: Xiebao capsule; Ferulic acid; HPLC Scorpion Capsule is a capsule developed in our hospital from Scorpio, angelica, myrrh etc., with enhanced immunity, to extend the survival of tumor-bearing mice the role of the clinical intended for use in adjuvant treatment of various tumors. In this study, using high performance liquid chromatography method in which the active ingredient content of ferulic acid were determined to control its quality. Are reported below. An instrument and reagent LC-10ATvp high-perfo