HPLC determination of flu season the content of the film Phillyrin.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of flu season the content of the film Phillyrin [Abstract] Objective Four Seasons by HPLC determination of the content of cold tablets Forsythin. Methods external standard point method, Diamonsil ODS1 C18 column, acetonitrile - water (25:75) as mobile phase, flow rate 1.0 mL / min, The detection wavelength was 277 nm. Results Forsythin in 0.112 8 ~ 0.902 4 μg range was a good linear relationship, the regression equation Y = 5.795 68X +1.843 19, r = 0.999 3, the average recovery was 98.6% , RSD = 1.82% (n = 6). Conclusion The method is simple, accurate, specific, and reproducible measurement results for the four quarters of cold tablets Phillyrin provides an effective quantitative analysis method. [Keywords:] Four Seasons cold tablets, phillyrin, HPLC, determination Abstract: Objective To establish the method for detemining the content of Phillyin in Siji Ganmao Pian by HPLC. Methods Diamonsil ODS1 C18 column was used with acetonitrile-water (25:75) as the mobile phase, detection wavelength as 277 nm and flow rate was 1.0 mL / min. Results The calibration curve was linear at the range of 0.1128 ~ 0.9024 μg for Phillyin and linear equation was Y = 5.795 68X +1.843 19, r = 0.999 3. The average recovery was 98.6% and RSD was 1.82% ( n = 5). Conclusion This method was simple, accurate and proper, the reduplication of the result was good. The method can be provide for quantitative analysis of Phillyin in Siji Ganmao Pian. Keywords:: Siji Ganmao Pian, Phillyin, HPLC, content determination Closed set out in the cold seasons films lt;lt;The Ministry of Health standards for traditional Chinese medicine prescription pharmaceutical preparationsgt;gt; Article 13, the function of relieving heat, cold cold for four seasons, especially for the infirm, pregnant women and cases of influenza caused by fever, headache, runny nose, cough, dry mouth, sore throat, nausea, anorexia and so on. prescribed by the basil leaves, Ne