HPLC determination of ethinyl estradiol liposome content and entrapment efficiency.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of ethinyl estradiol liposome content and entrapment efficiency Author: Chen Yun, Chen Tao, LU Ting-li, Hu Huijing, MA Yu-Fan [Abstract] Objective To determine the content of ethinyl estradiol and the liposomal encapsulation efficiency methods. Methods HPLC method in order to Agilent HC  C18 column (4.6 mm * 250 mm, 5 μ m) as column, mobile phase was methanol  water (volume ratio 70:30), column temperature at room temperature, flow rate of 1.0 mL * min -1, UV detection wavelength was 281 nm. The results of ethinyl estradiol and Lecithin, solvent peak can be effectively separated, ethinyl estradiol in the 2 ~ 20 μ g * mL-1 a good linear relationship scope (r = 0.999 7, n = 10), recoveries were 98.0% ~ 102.0% between. Conclusion The method is simple, accurate, high sensitivity method is reliable and can be used as ethinyl estradiol liposome content and encapsulation efficiency of quality control methods. [Keywords:] RP-HPLC; ethinyl estradiol liposomes; determination; entrapment efficiency Abstract: Objective To establish an HPLC method for the analysis of ethinylestradiol content and entrapment efficiency of ethinylestradiol liposomes.Method Samples were analyzed on an Agilent HC  C18 column (4.6 mm * 250 mm, 5 μ m) using methanol  water (70:30 ) as mobile phase. The flow rate was 1.0 mL * min-1 and detection wavelength 281 nm.Results Ethinylestradiol had a good linearity over the range of 2 ~ 20 μ g * mL-1 (r = 0.999 7, n = 10), the average recovery was between 98.0% and 102.0%. Conclusion This method is simple, accurate and sensitive for the quality control of the liposomes. Keywords:: HPLC; ethinylestradiol liposomes; ethinylestradiol; entrapment efficiency , Also known as ethinylestradiol ethinylestradiol for hydroxy-3  to  19  A  17α  pregnane  1,3,5 (10)  leukotriene  20  acetylene  17  alcohol. Chemical structure of [1]: Ethinyl estradiol as estrogen medicines, their