HPLC determination of furosemide zinc content of enema furazolidone.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of furosemide zinc content of enema furazolidone [Keywords:] furosemide zinc enema;,, furazolidone;,, zinc sulfadiazine;,, procaine hydrochloride;,, high-performance liquid chromatography Abstract: Objective: To determine zinc enema furazolidone furosemide concentration by high performance liquid chromatography. Methods: HYPERSIL ODS2 column (200 mm * 4.6 mm, 5 μ m), 0.033 mol / L potassium dihydrogen phosphate - acetonitrile (60:40) as mobile phase, flow rate of 1.0 ml / min, detection wavelength was 367 nm. Results: Furazolidone in the 1.016 ~ 8.128 μ g / ml range, the peak area with the linear relationship was good (r = 0.999 8), the average recovery was 99.0%, RSD 1.0% repeatability experiment. Conclusion: The method is accurate, simple, rapid and designed by the strong, high sensitivity, for furosemide zinc enema Determination of furazolidone. Keywords:: furosemide zinc enema; furazolidone; zinc sulfadiazine; procaine hydrochloride; high-performance liquid chromatography I made preparations furosemide zinc hospital enema mainly by furazolidone, sulfadiazine zinc, procaine hydrochloride drugs such as the composition of the enema with anti-bacterial, anti-inflammatory and promote healing of ulcers, clinical widely used in the treatment of ulcerative colitis , has good curative effect and the advantages of a short course of treatment. To control the quality of the preparation, we have established a high-performance liquid chromatography of main ingredients in this product furazolidone content. Are reported below. 1 Materials Furosemide zinc enema (hospital preparation room provided), furazolidone reference substance for the Chinese pharmaceutical and biological products to provide, acetonitrile, methanol HPLC pure, other reagents of analytical pure. 2 Methods and Results 2.1 Chromatographic conditions the United States DIONEX Summit HPLC, P680 pump, ASI-Autosampler, TCC-100 oven, UVD170 u detector, mobile phas