HPLC determination of compatibility of the drug in safflower Taoren HSYA A content changes.docVIP

 PAGE \* MERGEFORMAT 3 HPLC determination of compatibility of the drug in safflower Taoren HSYA A content changes Authors: Zhang, Wang Tao, Qi-De Liu, Chen Shaojie [Abstract] [Objective] To observe the different compatibility Peach Kernel Safflower ratio (mass ratio, respectively 1:1,1:2,1:3) in the decoction environment on HSYA A (HSYA) content of impact. [Methods] RP-HPLC (RP  HPLC) method, column was Luna C18 column (4  6 mm * 150 mm, 5 μ m), with methanol  0  12 mmol / L phosphoric acid (28: 72) as mobile phase, detection wavelength 403 nm, flow rate 1  0 mL / min, column temperature was 30 ℃ . [Results] HSYA in 4  0 ~ 160  0 μ g / mL concentration range a good linear relationship, the regression equation Y = 25 396X 40 247 (r = 0  999 7), the average recovery was 94  76% , the relative standard deviation (sR) for 2  93%. With the amount of the basic content of safflower HSYA ratio showed a corresponding increase exponentially, with no obvious relationship between dosage of peach kernel. [Conclusion] This study established HSYA method is simple and accurate, reproducible and can be used for medicinal safflower and its compound preparation of quality control standards, peach kernel of HSYA no significant effect on the dissolution. [Keywords:] peach kernel / chemistry; safflower / chemistry; HSYA A / analysis; chromatography, high pressure liquid Compositae safflower (Carthamus tinctorius L.) is a new oil-bearing crops, its dry Guanzhuang Hua called saffron (also known as grass saffron, safflower velvet, silk red flower, etc.), blood circulation is a traditional Chinese medicine. Modern pharmacological studies have shown that saffron has anti-coagulation, anti-thrombosis, blood pressure, blood fat and so on efficacy in the treatment of cerebral thrombosis, stroke, coronary heart disease, high blood pressure and other cardiovascular and cerebrovascular diseases, effect is significant [1-2]. Rose plant peach peach kernel for Prunus persi