HPLC determination of double yellow anti-inflammatory Berberine Hydrochloride.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of double yellow anti-inflammatory Berberine Hydrochloride [Abstract] Objective To establish a HPLC method for the determination of double yellow anti-inflammatory berberine hydrochloride capsules. Methods Dumas C18 (200 mm 4.6 mm, 5 m) as the analytical column, acetonitrile -0.05 mol / L sodium dihydrogen phosphate - triethylamine (26:74:0.5, adjusted with phosphoric acid to a pH value of 4.0) as mobile phase, flow rate: 1.0 mL / min, detection wavelength: 265 nm, determined by external standard. berberine hydrochloride injection volume results in the range of 0.02932 ~ 0.26388 g peak area was a good linear relationship (r = 0.9 999). The average recovery was 95.7%, RSD was 0.3% (n = 6). Conclusion The method is simple, accurate and reliable and suitable for the double yellow anti-inflammatory capsules determination of berberine hydrochloride. [Key Words] high performance liquid chromatography; double yellow anti-inflammatory capsules; berberine content; Abstract: Objective To establish an HPLC method for determination of the content of berberine hydrochloride in Shuanghuang Xiaoyan capsule. Methods The analysis column is Diamonsil C18 (200 mm 4.6 mm, 5m), the mobile phase is Acetonitrile-0.05 mol / L Sodium dihydrogen Phosphate-Triethylamine (26: 74: 0.5, and adjust pH to 4.0 with phosphoric acid), the flow velocity is 1.0 mL / min, the detection wavelength is 265 nm. External standard method is used for quantitative determination. Results Injecting sample quantity of berberine hydrochloride shows a good linear relationship with the peak area in the range between 0.02 932 g and 0.26 388 g (r = 0.9 999). The average recovery rate is 95.7%, and RSD is 0.3% (n = 6). Conclusions The method is simple, rapid, accurate and reliable. It is suitable for determination of the content of berberine hydrochloride in Shuanghuang Xiaoyan capsule. Keywords:: HPLC (high performance liquid chromatography); Shuanghuang Xiao