HPLC Determination of Compound Yimu oral solution content stachydrine.docVIP

 PAGE \* MERGEFORMAT 9 HPLC Determination of Compound Yimu oral solution content stachydrine [Abstract] Objective To establish a high performance liquid chromatography oral solution compound Yimu stachydrine content. Methods Kromasil C18 (5 m, 250 mm 4.6 mm) for the column, acetonitrile 0.05 mol L-1 phosphate potassium hydroxide solution (volume ratio of 18:82, pH value of 5.5 as the mobile phase, flow rate 1.0 mL min-1, column temperature 35 , detection wavelength 215 nm, external standard method for testing. Results stachydrine injection volume of 1.00 ~ 25. 00 g range with good linearity, r = 0.999 7, the sample average recovery was 98.1% (n = 9), RSD is 1.17%. Conclusion The method is simple, accurate and reliable, reproducible, and can be used to control the quality of preparation methods. [Keywords:] stachydrine HPLC Liquid Compound Yimu Motherwort is a traditional gynecological medicine, sex spicy, bitter, slightly cold, with blood stasis, the effect of menstruation and Water, commonly used in women, irregular menstruation, dysmenorrhea, dystocia, and bruises, swelling and sore swollen poison embolism. pharmacological study found that Motherwort Leonurus alkali, water, alkali and other active alkaloids of the Soviet Union also has to improve myocardial ischemia, increased coronary blood flow, improve cardiac function, inhibit platelet aggregation, anticoagulant, anti-thrombosis a variety of roles, and the lasting effect [1]. Compound Yimu oral pills by Yimu (China Pharmacopoeia formulations change from 1977 in which the main active ingredient is Leonurus stachydrine Alkaloid determination of index components, more than reported in the literature using UV spectrophotometry TLC and high performance capillary electrophoresis to determine not only the complicated operation required a long time, but Stachydrine stability when the humidity is not ideal [2-5]. this compound by high performance liquid chromatography The main active ingredients of