Huang TF ischemia-reperfusion myocardial apoptosis and apoptosis-related gene expression in.docVIP

 PAGE \* MERGEFORMAT 7 Huang TF ischemia-reperfusion myocardial apoptosis and apoptosis-related gene expression in Author: Gong Mingyu, at Xiao-Min, Zhou Xiaohui, YANG He-enzyme [Abstract] Objective To observe the Huang TF (Scutellaria Baiculensis Stem-leaf Total Flavonoid, SSTF) on ischemia-reperfusion injury in rat cardiac cell apoptosis and apoptosis-related gene expression. Methods ligating the left anterior descending coronary artery 30 min, and then release the 2 h reperfusion method, replication of rat myocardial ischemia-reperfusion injury model. Rats were divided into sham-operation group, ischemia-reperfusion group (IR), SSTF different dose treatment group. In situ nick end labeling (TUNEL) and immunohistochemical staining to detect myocardial apoptosis index (AI), Bax and Bcl-2 gene protein expression. The results compared with the sham-operated group, ischemia-reperfusion group AI increased, Bax expression increased, Bcl-2 expression decreased (P amp;lt;0.01); with ischemia-reperfusion group, SSTF group AI, Bax expression decreased (P amp;quot;0.05 or P amp;lt;0.01), Bcl-2 expression increases (P amp;lt;0.05 or P amp;lt;0.01). Conclusion SSTF significantly inhibited the IR-induced myocardial apoptosis, and its mechanism may be related to reduced Bax protein expression and increase Bcl-2 protein expression and improve the ratio of Bcl-2/Bax a certain extent. [Keywords:] Huang TF-reperfusion injury in apoptosis gene expression in Abstract: ObjectiveTo observe the effects of total flavonoid (SSTF) in Scutellaria Baiculensis Stem-leaf on myocyte apoptosis and the expression of apoptosis-associated genes in rat hearts with acute myocardial ischemia and reperfusion (IR). MethodsThe left anterior descending branch of coronary artery was ligated for 30 min and reperfused for 2h to make a myocardial ischemia-reperfusion model in rats.The experiment rats were divided into five groups: sham, ischemia-reperfusion (IR), SSTF (17.5,35,70 mg * kg-1 * d-1) gr