Identification of ISSR markers using authentic ingredients of the orange-red.docVIP

 PAGE \* MERGEFORMAT 13 Identification of ISSR markers using authentic ingredients of the orange-red [Abstract] Objective To explore the use of ISSR molecular markers identify the molecular level in the nucleic acid of orange genuine medicine. Methods from 20 ISSR primers of the appropriate primers, orange, orange, grapefruit and other 16 samples were PCR amplified and electrophoresis analysis, looking for characteristics of sites. The results are two ISSR primers polymorphism of the more obvious bands, individually or in combination, identification of the orange. Conclusions ISSR as a simple and reliable molecular markers that can be used in the identification of an orange-red. [Keywords:] of the orange-red, ISSR, molecular markers Abstract: Objective To identify Exocarpium Citri Grandis by inter-simple sequence repeat markers. Methods Primers suitable for routine analysis were screened from 20 inter simple sequence repeat primers, then, they were used in PCR and sepharose electrophoresis of 16 samples of Exocarpium Citri Grandis, Citrus reticulata and Citrus grandis (Linn.Osbeck.Results Two of the 20 primers amplified polymophic bands and suitable for the identification of Exocarpium Citri Grandis. Conclusion Inter simple sequence repeat markers provide a quick, reliable molecular marker for identification of Exocarpium Citri Grandis. Keywords:: Exocarpium Citri Grandis; inter simple sequence repeat; identification Of orange-red (Exocarpium Citri Grandis is grapefruit Rutaceae Citrus grandis (L.) Osbeck immature or nearly mature dry outer layer of skin, commonly known as orange peel, of the state orange, grapefruit skin. Cultivars of Citrus grandis Citrus grandis Citrus grandis “Tomentosa” for the outer skin of the orange with the medicine. medicine was a seven-fold, or expand the five-pointed star-shaped angle, single-chip was willow-shaped, air aromatic, bitter, slightly acrid, with a cold, dampness, Li Qi, Phlegm and other effects. mainly pr