Hypoglycemic mechanism of action of tea polysaccharide.docVIP

 PAGE \* MERGEFORMAT 8 Hypoglycemic mechanism of action of tea polysaccharide [Abstract] Objective To study the mechanism of hypoglycemic effect of tea polysaccharides. Methods colorimetric determination of tea polysaccharides on α  glucosidase (EC 3  2  1  20), and α  amylase (EC 3  2  1  1) of the inhibitory activity was observed with rapid filtration of tea polysaccharide of rabbit intestinal brush-border vesicle glucose transport activity. The results show a strong tea polysaccharide α  Glucosidase inhibitory activity, its α  Glucosidase half-inhibitory concentration (IC50) value of 3  2g / L; tea polysaccharides on α  amylase inhibitory activity was relatively weak, concentration of 1g / L, its right α  amylase inhibitory rate of 10%; tea polysaccharides while significantly reducing the rabbit intestinal brush-border vesicle glucose transport capacity, the half-inhibitory concentration (IC50) value of 15g / L. Conclusion of tea polysaccharides inhibit glucosidase enzyme α  α  amylase and intestinal brush-border vesicle glucose transport capacity, which may delay the intestinal absorption of sugar. [Keywords:] Tea Polysaccharide Tea is a kind of polysaccharide extracted from the tea leaves to the multi-sugar compounds, with enhanced immunity, lowering blood pressure, lowering blood sugar, anti-radiation, anti-clotting, anti-thrombotic effects, etc. 〔 1,2〕 . China and Japan, the old folk have Paoyin rough treatment of diabetes, history of tea 〔 3〕 . Preliminary study confirmed that the polysaccharide is a rough old tea tea hypoglycemic main active ingredient 〔 4〕 , but the specific mechanism of action is unclear. In this paper, tea polysaccharide as the material to explore its α  Glucosidase, α  amylase and brush border vesicle glucose transport capacity of inhibition to reveal the hypoglycemic mechanism of tea polysaccharides. 1 Materials and methods 1  1 reagents and equipment of yeast α  Glucosidase (J