Huoxue Capsules on rat brain ischemia-reperfusion after focal cerebral protective effect of nerve cells.docVIP

 PAGE \* MERGEFORMAT 19 Huoxue Capsules on rat brain ischemia-reperfusion after focal cerebral protective effect of nerve cells [Abstract] [Objective] To study the blood circulation Brain capsule on cerebral ischemia-reperfusion nerve cell protection. [Method] selected SD rats were randomly divided into sham operation group, model group, nimodipine group (dose of 10.8mg * kg-1 * d-1), activating blood brain capsule group (dose of 1.8g * kg - 1 * d-1), further divided into 3 groups after 2h ischemia and reperfusion 6,12,24 h group; used to copy suture method in rats with focal cerebral ischemia-reperfusion model, immunohistochemical detection neurons cytochrome C (Cyt C), in situ hybridization detection of Caspase  3mRNA, Caspase  9mRNA expression. [Results] Huoxue brain capsule group cerebral ischemia / reperfusion 6 h weak positive expression of cytochrome C, compared with the model group were significantly different (P amp;lt;0.01), cerebral ischemia / reperfusion 12h, 24h cytochrome C, positive expression in the model group and sham operation group showed no significant difference (Pamp;gt; 0.05). Model of cerebral ischemia-reperfusion 24h hippocampus Caspase  3 mRNA, Caspase  9mRNA expression peaked (P amp;lt;0.01); Huoxue brain capsule cerebral ischemia / reperfusion 24hCaspase  3 mRNA, Caspase  9mRNA expression weakened, Compared with model group were significantly different (P amp;lt;0.01). [Conclusion] The blood circulation to the brain capsule after focal cerebral ischemia-reperfusion of the protective effect of nerve cells can affect the nerve cells may be related to the release of Cyt C and reduce cerebral ischemia / reperfusion brain tissue Caspase  3, Caspase  9 of the activity, thereby blocking related to the occurrence of apoptosis. [Keywords:] Huoxue brain capsule / pharmacology; cerebral ischemia / TCD therapy; brain tissue / pathology; apoptosis; disease models, animal; rat 1 Materials and methods 1.1 Animals and drug