Human embryonic stem cell research.docVIP

 PAGE \* MERGEFORMAT 25 Human embryonic stem cell research [Keywords:] human embryonic stem cells; Application Stem cells (Stemcell) are a class of self-renewal capacity of pluripotent cells, ie stem cells remain undecided differentiation state and have proliferative capacity, in appropriate conditions or give the appropriate signal, which can differentiate into a variety of functional cells or tissues and organs , also known as a “wildcard cells.” stem cells derived from embryos, fetal tissues and adult tissues. According to developmental stage, the stem cells into embryonic stem cells and adult stem cells In 1998 Thomson et al (1) for the first time from the embryo isolation and culture of the human embryonic stem cells (human embryonic stem cell, hESC), and then found it to differentiate into almost all the cells in vivo, which set off a worldwide craze hESC research. In this paper, human embryonic stem cells as well as the basic theory of human embryos at home and abroad stem cells research and clinical applications are reviewed. A source of human embryonic stem cells Embryonic stem cells derived from preimplantation blastocyst inner cell mass (Inter cell mass, ICM) or primordial germ cells of early embryos (Embryonic primordial germ cell, EG), is a large class of undifferentiated diploid totipotent cells that have unlimited proliferation, self-renewal and multilineage differentiation potential In 1998, Thomson et al (1) first reported the person 5 d in vitro fertilization blastocysts successfully isolated human embryonic stem cells (Human embryonic stem cell, hESC), in vitro maintain their undifferentiated state were passaged 30 generations or more. In the same year, Shamblott (2) reported from the fertilized 5 ~ 9 w of the human embryo contains the primordial germ cells, genital ridge and mesentery successfully isolated and cultured more than five potential stem cell lines, the characteristics similar to embryonic stem cells, continuous passag