HPLC Shichimi safflower Most Excellent Pill gallic acid content.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Shichimi safflower Most Excellent Pill gallic acid content [Abstract] Objective To determine the Shichimi safflower Most Excellent Pill gallic acid method. Methods kromasil-C18 (4.6 mm * 250 mm, 5 μ m) column; mobile phase was methanol: water (containing 0.04% phosphoric acid) = five ninety-five; detection wavelength was 274 cm; flow rate 1 ml * min-1; column temperature 35 ℃ . The results of gallic acid 0.010 3 ~ 0.206 μ g showed a good linear relationship between the. The average recovery was 100.32%, RSD = 1.88% (n = 6), r = 0.999 8. Conclusion The method is simple, high sensitivity, good reproducibility and can be used as the preparation of quality control methods. [Keywords:] high-phase liquid chromatography Shichimi safflower Most Excellent Pill gallic acid Qiwei Tibetan safflower Most Excellent pill is often used, in order to tan Shuiwan, gas and slightly sweet smell, bitter taste. Major components of safflower, Tianzhu Huang, Swertia, myrobalan, ephedra, costas, Aristolochia, five pulse Meconopsis. Anti-inflammatory function of heat, nourish the liver and back yellow, for the old and new liver injury caused by hepatic blood by labor Sheng, hepatomegaly, sclera yellow staining and loss of appetite [1]. Which contains a large number of gallic acid. Determination of gallic acid by TLC-scanning method [2,3], high performance liquid chromatography [4,5]. Order to effectively control its quality, this paper, high-performance liquid chromatography Shichimi safflower Most Excellent Pill gallic acid content was determined by receipt of satisfactory results. The method is simple, reliable, good separation results are stable, in order to control the quality of products provide a reliable basis. An apparatus and materials Agilent 1100 HPLC, configuration: manual injector, on-line Degasser, efficient binary gradient pump, constant temperature oven, DAD detector, Agilent 1100 chromatography workstation. Gallic acid reference substa