HPLC simultaneous determination of cefalexin and plasma concentration of trimethoprim.docVIP

 PAGE \* MERGEFORMAT 13 HPLC simultaneous determination of cefalexin and plasma concentration of trimethoprim Author: Xiadeng Ning Xia Yan Jiao Yin Jia Ying-Ju [Abstract] using high-performance liquid chromatography UV detector simultaneous detection of plasma cefalexin and trimethoprim concentration. To 0.5 times the plasma volume, the concentration of 10% (v / v) in perchloric acid as the protein precipitant. Chromatographic conditions: Diamonsil C18 (250mm * 4.6mm, 5μ m), column temperature: 30 ℃ , mobile phase: pH3.0 in 0.05mol / L phosphate buffer: acetonitrile: methanol (76:16:8), flow rate 1.0 ml / min, injection volume of 20μ l, detection wavelength 240nm. Cefalexin, trimethoprim, and a good separation of endogenous substances. Cephalexin serum concentration linear range of 0.5 ~ 50.0μ g/ml, detection limit of 50ng/ml, high, medium and low concentration of the relative recoveries of 97.3% ~ 100.5% for (n = 5), day RSD ≤ 6.5% (n = 5), day RSD ≤ 8.3% (n = 5). Trimethoprim plasma concentration of linear range of 0.25 ~ 25.0μ g/ml, detection limit of 25ng/ml, high, medium and low concentration of the relative recoveries of 96.5% ~ 99.3% for (n = 5), days RSD ≤ 6.9% (n = 5), day RSD ≤ 8.6% (n = 5). Law sample handling simple, sensitive, accurate, and suitable for simultaneous determination of both the concentration in vivo. [Keywords:] cephalexin; trimethoprim; blood drug concentration; high performance liquid chromatography. ABSTRACT To establish an HPLC method for the simultanecous determination of cefalexin and trimethoprin in rabbits’ plasma. The protein was precipitated by perchloric acid. Chromatographic separation of analytes was achieved on a C18 column at the temperature of 30 ℃ , using a mixture of 0.05mol / L PBS (pH = 3.0), acetonirile and methanol (76:16:8, v / v / v) as mobile phase and detection was performed at 240 nm. The linearity was obtained over the concentration range of 0.5 ~ 50.0μ g / ml for cefalexin and 0.25 ~ 25.0μ g/ml for