HPLC method for the determination of quercetin in Pyrola content_0.docVIP

 PAGE \* MERGEFORMAT 7 HPLC method for the determination of quercetin in Pyrola content Of: Zhang, Li Yu Ling, Feng Li Bin, Fan Weifeng [Abstract] Objective To establish a HPLC method for the determination of quercetin in Pyrola content. Methods using HPLC, Column: Agilent Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm), mobile phase: methanol -0.1 % phosphoric acid (50:50), detection wavelength: 370 nm. the results of quercetin in the range of 0.092 ~ 0.46 μg good linear relationship, the average recovery was 99.24%, RSD = 0.70%. Conclusion The method is simple, accurate, high sensitivity, reproducible, effective medicines in the determination of Pyrola quercetin. [Keywords:] Pyrola, quercetin, HPLC, determination Abstract: Objective To set up a method for determining the quercetin in Pyrola calliatha H. Andres. Method HPLC method was carried on Agilent Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm) with mobile phase of methanol-0.1% phosphoric acid (50:50), and the detecting wavelength was at 370 nm. Result The standard curve was linear within the range of 0.092 ~ 0.46 μg. The average recovery rate was 99.24% and RSD was 0.70%. Conclusion The method is simple, accurate, highly sensitive and reproducible. It can be used for the quality determination of quercetin in Pyrola calliatha H. Andres. Keywords:: Pyrola calliatha H. Andres, quercetin, HPLC, content determination Pyrola Pyrola woody plant as Pyrola Pyrola calliatha H. Andres dry the whole plant, often called deer grass title, published in 2005 Edition lt;lt;Chinese Pharmacopoeiagt; “(a). Its warm, taste joys, with rheumatism, strong bones and muscles, the effect of bleeding for Fengshibitong, waist and knee weakness, menorrhagia, chronic cough cough workers [1]. modern pharmacological studies have shown that the drug has significant anti-bacterial, anti-inflammatory to promote immunization, improve the role of coronary heart disease symptoms [2]. The main contains Pyrolin, quercetin an