HPLC method for the determination on the injection Qingkailing content hyodeoxycholic.docVIP

 PAGE \* MERGEFORMAT 9 HPLC method for the determination on the injection Qingkailing content hyodeoxycholic [Abstract] Objective To establish Qingkailing hyodeoxycholic injection method for the determination. Methods HPLC, column was UltimateXB-C18 (5 μm, 250 mm × 4.6 mm), mobile phase of acetonitrile - water - phosphoric acid (35:65:0.1), flow rate 1 mL / min, column temperature was 40 ℃, the detection wavelength was 192 nm. hyodeoxycholic results in 0.201 35 ~ 1.006 75 mg / mL range a good linear relationship, r = 0.999 6, the average recovery was 98.25%, RSD = 1.26%. Conclusion The method is accurate, reliable, reproducible and can be used for injection Qingkailing quality control. [Keywords: high performance liquid chromatography, Qing Kai Ling Injection, hyodeoxycholic Abstract: Objective To establish a method for content determination of hyodeoxycholic acid in Qingkailing Injection. Methods An UltimateXB-C18 column (5 μm, 250 mm × 4.6 mm) was used with a mobile phase of acetonitril-water-phosphoric acid (35:65 : 0.1). The flow rate was 1 mL / min. The temperature of the column was 40 ℃. The detection wavelength was 192 nm. Results There was a good linear relationship between the concentration of hyodeoxycholic acid and absorption area value in range of 0.201 35 ~ 1.006 75 mg / mL, r = 0.999 6. The average recovery was 98.25% with RSD = 1.26%. Conclusion This method was accurate, credible and repeatable which can be used to control the quality of Qingkailing Injection. Keywords:: HPLC, Qingkailing Injection; hyodeoxycholic acid Qingkailing Angongniuhuang improved compound for the injection, with heat and detoxifying, phlegm meridians, Stasis resuscitation of power, for fever, coma, stroke, paralysis, unconsciousness, can be used for acute and chronic hepatitis, B, upper respiratory tract infection, pneumonia, high fever and cerebral thrombosis, cerebral hemorrhage syndrome who see the above. bezoar in its original prescription has the perve