HPLC method for the determination on the worn red stones of emodin.docVIP

 PAGE \* MERGEFORMAT 8 HPLC method for the determination on the worn red stones of emodin [Abstract] Objective To establish a red stone worn Emodin high performance liquid chromatography method. Methods Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm), methanol -0.1% phosphoric acid (75: 25) as mobile phase, detection wavelength was 285 nm. Results of emodin in the range of 0.0063 ~ 0.063 μg showed a good linear relationship (r = 0.999 9), the average recovery was 102.0%, RSD = 1.58% (n = 6 ). Conclusion The method is simple, accurate, and can be used for red rock pierced the determination of emodin. Keywords:] red worn stone, emodin, high performance liquid chromatography Abstract: Objective To establish a HPLC method for determining emodin in Radix et Caulis Ventilaginis leiocarpae. Methods Stationary phase was Agilent Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm), mobile phase was methanol-0.1% phosphoric (75:25 ), detection wavelength was 285 nm, flow rate was 1.0 mL / min and column temperature was 30 ℃. Results There was good linearity in the range of 0.006 3 ~ 0.063 μg for emodin (r = 0.999 9). The average recovery of emodin was 102.0% with RSD = 1.58%. Conclusion This method is rapid and accurate. It can be applied to control the quality of Radix et Caulis Ventilaginis leiocarpae. Keywords:: Radix et Caulis Ventilaginis leiocarpae, emodin, HPLC Worn stone red wing nut Rhamnaceae plants Ventilago leiocarpa Benth.’s Dried roots and old stems, mainly produced in Guangxi, [1], with heat and dampness, diuretic Tonglin role, in particular stone has a special cancellation effect, civil commonly used in urinary tract infection, acute and chronic nephritis, kidney stones treatment, is now home to several pharmaceutical treatment of stone disease as an important group of parties. Wang et al [2] reported that production of red from the Guangxi worn stone isolated from the roots and stems of emodin, emodin methyl ether, 1,2,4,8 - tetrahydroxy -3-- methyl ant