HPLC-ELSD determination of refreshing recycling capsule contents Astragaloside_0.docVIP

 PAGE \* MERGEFORMAT 8 HPLC-ELSD determination of refreshing recycling capsule contents Astragaloside [Abstract] Objective To establish a refreshing recycling astragaloside capsule method for the determination. Methods HPLC-ELSD method, Phenomenex Gemini C18 column (250 mm × 4.60 mm, 5 μm), mobile phase: acetonitrile - water (36: 64), flow rate: 1.0 mL / min, drift tube temperature: 105 ℃, carrier gas flow rate: 2.7 L / min. Results astragaloside range of 0.486 ~ 2.43 μg linear relationship, r = 0.999 7, the average recovery was 100.5% (n = 6), RSD = 0.3%. Conclusion The method is accurate, sensitive, reproducible, and can be used as the quality of the drug testing standards. [Keywords:] refreshing recycling capsules, astragaloside, high performance liquid chromatography - evaporative light scattering detection method, determination Abstract: Objective To establish a quantitative method for Astraloside in Xingnao Zaizao Capsules by HPLC-ELSD. Methods HPLC-ELSD separation was achieved on Phenomenex Gemini C18 column (250 mm × 4.60 mm, 5 μm) and mobile phase consisted of acetonitrile-water ( 36:64). The flow rate was 1.0 mL / min, drift tube temperature was 105 ℃, and gas flow rate was 2.7 L / min. Results The calibration curve was linear in the range of 0.486 ~ 2.43 μg (r = 0.999 7 ). The average recovery rate was 100.5% (n = 6), RSD was 0.3%. Conclusion The method is rapid, accurate and reliable, and the method can be used as the quality standard for Xingnao Zaizao Capsules. Keywords:: Xingnao Zaizao Capsules, Astraloside, HPLC-ELSD, content determination Refreshing recycling capsule collection is contained in lt;lt;The Ministry of Health standards for traditional Chinese medicine prescription pharmaceutical preparationsgt;gt; the first four, with phlegm refreshing, Qufeng active effect. For the confusion, language Jian astringent, mouth salivation, kidney paralysis, myalgia, foot Spasm, hemiplegia and cerebral thrombosis in convalescence and seq