HPLC was used to determine the origin senticosus chlorogenic acid and hyperin content_0.docVIP

 PAGE \* MERGEFORMAT 11 HPLC was used to determine the origin senticosus chlorogenic acid and hyperin content Of: Shao Jing, Di Liu-qing, Xie new, Dong Yu, Xiao-Li Zhao, Wu Hao Xin, Liu Ming products [Abstract] Objective To establish the ASS and chlorogenic acid in determining the content of hyperin, we determined the origin of ASS and chlorogenic acid content of hyperin. Methods Hedera · ODS-2 C18 column (250 mm × 4.6 mm, 5 μm), acetonitrile -0.4% phosphoric acid (17:83) as mobile phase, detection wavelength was 360 nm. Results of chlorogenic acid and the concentration of hyperin at 10.2 ~ 306 μg / mL (r = 0.999 5) and 10.4 ~ 312 μg / mL (r = 0.999 8) between the peak area and showed a good linear relationship, the average recoveries were 97.14% and 98.21%, RSD 2.65% respectively and 1.89%. different areas the amount contained in the sample were quite different. Conclusion The method is simple, accurate with good repeatability for the further development and application of ASS and laid the foundation for quality control [Keywords:] senticosus, chlorogenic acid, hyperin, HPLC, determination Abstract: Objective To establish a HPLC method for the content determination of Chlorogenic acid and Hyperoside in the leaves of acanthopanax senticosus from different areas. Methods The samples were separated on Hedera · ODS-2 C18 column, using acetonitrile-0.4% phosphoric acid (17 : 83) as mobile phase, the detection wavelength was 360 nm. Result Chlorogenic acid and Hyperoside showed good linear relationship over the ranges of 10.2 ~ 306 μg / mL (r = 0.999 5) and 10.4 ~ 312 μg / mL (r = 0.999 8), respectively. The average recoveries were 97.14% and 98.21% with RSD at 1.65% and 1.09%, respectively. The contents of Chlorogenic acid and Hyperoside were different obviously in samples from different areas. Conclusion This method is simple, rapid and sensitive , and it can be used for the further development and the quality control of the leaves of acanthopanax sent