HPLC-DAD method for simultaneous determination of Dachuanxiong ferulic acid compound in the drug delivery system and the content of gastrodin.docVIP

 PAGE \* MERGEFORMAT 10 HPLC-DAD method for simultaneous determination of Dachuanxiong ferulic acid compound in the drug delivery system and the content of gastrodin [Abstract] Objective DAD detector can simultaneously detect multiple absorption-line strengths, can be determined simultaneously to establish a drug delivery system Dachuanxiong compound ferulic acid content and gastrodin RP-HPLC. Methods ODS-2 Hypersil column (250 mm × 4.6 mm, 5 μm), methanol -1% glacial acetic acid as mobile phase, gradient elution, flow rate 1 mL / min, column temperature 25 ℃, Gastrodin detection wavelength 270 nm, ferulic acid detection wavelength 322 nm. Results gastrodin 40 min with ferulic acid and other components within the can achieve good separation, Gastrodin range of 0.092 ~ 1.840 μg showed a good linear relationship, r = 1.000 0, ferulic acid in the range of 0.122 ~ 2.440 μg showed a good linear relationship, r = 1.000 0. gastrodin recovery was 99.50%, RSD = 1.23% (n = 5), ferulic acid recovery was 101.5%, RSD = 1.52% (n = 5). Conclusion The method is simple, accurate and reliable, simultaneous determination of drug delivery systems Dachuanxiong compound ferulic acid and gastrodin two effective components. [Keywords:] Dachuanxiong compound drug delivery system, gastrodin, ferulic acid, HPLC / diode array detector, determination Abstract: Objective To set up a RP-HPLC method for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously. Methods ODS-2 Hypersil column was used with methanol-1% glacial acetic acid as solution gradient elution. The flow rate was 1 mL / min and column temperature was 25 ℃. The wavelength of detector of gastrodin was 270 nm, and ferulic acid was 322 nm. Results Gastrodin and ferulic acid can be separated well with other components within 40 minutes. The linear range of gastrodin was 0.092 ~ 1.840 μg (r = 1.000 0), and ferulic acid was 0.122 ~ 2.440 μg (r = 1.000 0). The average recovery rate of g