Human embryonic muscle cells in vitro spontaneous malignant transformation of changes in tumor suppressor genes.docVIP

 PAGE \* MERGEFORMAT 13 Human embryonic muscle cells in vitro spontaneous malignant transformation of changes in tumor suppressor genes [Abstract] Objective: To study the muscles of human embryos derived spontaneously transformed cell lines of malignant tumor suppressor genes. Methods: Using immunohistochemistry, PCR, PCR product direct sequencing study of such cell line tumor suppressor gene p16, p15, MTAP (methionine phosphorylase) and the p53 gene and protein changes. Results: The chromosome 9p21 that there exist homozygous deletion fragment, including p16, p15, and MTAP; on the high rate of p53 gene mutation in exon 7,8 and intron 7 sequence is found a wider range of mutations in exon mutation rate as high as 13% are all run point mutations, while the intron variation even worse, including small fragment deletion, insertion, translocation and point mutation, which is almost a normal p53 sequence had no significant similarity in the sequencing of the six cell lines China (including five different genetic background) mutation sequences have a high degree of consistency. Conclusion: The multiple tumor suppressor gene mutation in normal spontaneous malignant transformation of embryonic muscle cells plays an important role in the process. [Keywords:] malignant sarcoma transforming gene chromosome 9p21 p53 tumor suppressor gene mutation 0 Introduction Inactivation of tumor suppressor gene or mutation will have a product of the proliferation of cell cycle regulation and apoptosis in impact interfere with the normal cycle, the process of malignant transformation of cells, providing a potential genetic basis. Among them, relatively few recognized the impact of the malignant transformation of cells in a large gene cyclin-dependent kinase inhibitor family of CDKI, Rb, p53, etc. [1,2]. At present, the spontaneous in vitro malignant transformation of human cells are few reports [3-7], recent studies have found that long-term in vitro culture of mesenchymal stem ce