HPLC method for the determination on the Garcinia cambogia Garcinia acid and acid content in the new.docVIP

 PAGE \* MERGEFORMAT 8 HPLC method for the determination on the Garcinia cambogia Garcinia acid and acid content in the new Of: Zhou An, Li Qinglin, Peng on behalf of the silver, Wu Hongfei, DONGTING, Wang Shan [Abstract] Objective To establish the Garcinia cambogia Garcinia acid and a new method for the determination of acid. Methods RP-HPLC column was Hypersil ODS C18 (4.6 mm × 250 mm, 5 μm), mobile phase: methanol - 0.1% acetic acid (93:7), flow rate 1.0 mL / min, detection wavelength was 360 nm, external standard. Results Gambogic acid linear range 2.45 ~ 49 μg, the regression equation Y = 11 334.8 +232 781X ( r = 0.999 9, n = 5), the average recovery rate of 99.3%, RSD = 1.02% (n = 6), linear range of the new gambogic acid 2.55 ~ 51 μg, the regression equation Y = 75 197.5 +226 301X (r = 0.999 7, n = 5), average recovery 100.5%, RSD = 1.48% (n = 6). Conclusion The high precision, good reproducibility, simple, reliable, and can be used as quality control of Garcinia. [Key Words] high performance liquid chromatography, Garcinia, Garcinia acid, a new gambogic acid, determination Keywords:: HPLC, Gamboge, Gambogic acid, Gambogenic acid, Content determination Department of Garcinia Garcinia cambogia Garcinia hanburyi Hook.f. plants secreted by the dry resin, cold, Pickle, Sim, Shibuya, toxic, with Poxue Sanjie, detoxification, bleeding, the effectiveness of insecticide for the treatment of scrofulous, ulcer, boil, swelling and other ills. the past 20 years found that Garcinia acid, a new gambogic acid has significant anti-tumor activity, particularly its emphasis on medical workers. at home and abroad, especially China scholars and activity Garcinia Garcinia cambogia acid composition and a new method for the determination of acid in a lot of research work, most of them by TLC method and HPLC-ELSD method [1-2]. In order to further improve the quality control of Chinese medicine Garcinia, this test uses HPLC -DAD method for simultaneous Gambogic