Human DNA repair gene HOGG1 eukaryotic expression vector and sequencing of.docVIP

 PAGE \* MERGEFORMAT 12 Human DNA repair gene HOGG1 eukaryotic expression vector and sequencing of Authors: WEI long, Nancke Jun, Hui-Ling Yun, Da-lin [Keywords:] Genetic Vectors; HOGG1 gene; Cloning, Molecular; DNA repair Construction and sequencing of eukaryotic expression vector of human DNA base excision repair gene HOGG1 [Abstract] AIM: To clone human DNA base excision repair gene, 8oxoguanineDNA glycosylase (HOGG1), and construct its eukaryotic expression vector with sequence analysis. METHODS: Total RNA was extracted from the liver tissue of human foetus by the guanidinium thiocyanate method. The whole HOGG1 coding gene was amplified by RTPCR and cloned into eukaryotic expression vector pCMVMyc. Plasmid DNA was extracted and subjected to restriction enzyme analysis. Clones representing cDNA inserts were sequenced. RESULTS: The construction of eukaryotic expression vector pCMVMyc / HOGG1 was successful and the resulted sequence completely coincided with the designs. CONCLUSION: The construction of eukaryotic expression vector pCMVMyc / HOGG1 provides a new way for biological targeted therapy involving DNA base excision repair gene HOGG1. [Keywords:] genetic vectors; HOGG1; cloning, moleculor; DNA repair [Abstract] Objective: To clone human HOGG1 genes and construct its eukaryotic expression vector for sequencing. Methods: The extract of human fetal liver total RNA, designed to HOGG1 specific primers were amplified by RTPCR, will HOGG1 gene was cloned into pCMVMyc vector, colony PCR positive clones identified and sequenced verified. Results: The full-length human HOGG1 gene, and successfully constructed the eukaryotic expression vector pCMVMyc/HOGG1. Conclusion: HOGG1 eukaryotic expression vector for genetically modified organisms HOGG1 the development of targeted therapies to provide a new tools. [Keywords:] Genetic Vectors; HOGG1 gene; Cloning, Molecular; DNA repair 0 Introduction Cells in the process of aerobic metabolism and exposure to enviro