HPLC-ELSD method for the determination on blood oral gelatin content of astragaloside.docVIP

 PAGE \* MERGEFORMAT 9 HPLC-ELSD method for the determination on blood oral gelatin content of astragaloside Of: Liu Tong Xiang, Shangbao Chang, Chen Fujun, Wang bee [Abstract] Objective To establish Ejiao Astragaloside blood oral solution method for the determination. Methods HPLC-ELSD method, column: Betasil C18 (4.6 mm × 200 mm, 5 μm), mobile phase: methanol - water (74: 26), flow rate: 1.0 mL / min, column temperature: 30 ℃, ELSD parameters: drift tube temperature of 90 ℃, gas flow 2.0 L / min. Results astragaloside 0.43 ~ 2.15 μg showed a good linear relationship between the regression equation: Y = 1.536 37X +0.921 58, r = 0.999 9 (n = 6), the average recovery 97.98%, RSD = 1.03%. Conclusion The method is accurate, reliable, reproducible and can be used as oral Ejiao blood Liquid quality control. [Keywords: HPLC-ELSD, Ejiao blood oral solution, astragaloside, determination Abstract: Objective To establish a method for the determination of Astragaloside Ⅳ in Ejiao Buxue Koufuye. Methods HPLC-ELSD was used to determine directly Astragaloside IV in Ejiao Buxue Koufuye on Betasil C18 column, using CH3OH-H2O (74:26) as a mobile phase. The flow rate was 1.0 mL / min. The temperature of drift tube for ELSD was 90 ℃ and the flow rate of N2 was 2.0 L / min. Results The linear range of Astragaloside Ⅳ was 0.43 ~ 2.15 μg, Y = 1.536 37X +0.921 58, r = 0.999 9 (n = 6), the average recovery of the added sample was 98.5% and RSD = 0.98%. Conclusion The method is accurate and reliable with a good reproducibility. It can be used for the quality control of Ejiao Buxue Koufuye. Keywords:: HPLC-ELSD, Ejiao Buxue Koufuye, Astragaloside Ⅳ, determination Ejiao blood oral solution for the traditional Chinese medicine, from Astragalus, donkey-hide gelatin, Rehmannia glutinosa, Codonopsis, wolfberry fruit, Atractylodes composition, with a yin blood , Bu Zhong Yi Qi, spleen and lungs of power, for Jiubingtixu, blood loss, dizziness, asthenia cough embolism [1]. astragal