Human cytomegalovirus immediate early smooth muscle cells after infection and late gene expression and gene regulation.docVIP

 PAGE \* MERGEFORMAT 11 Human cytomegalovirus immediate early smooth muscle cells after infection and late gene expression and gene regulation [Abstract] Objective To detect the in vitro smooth muscle cell (SMCs) infected with human cytomegalovirus (HCMV) immediate early after (IE) gene and late (L) gene expression and regulation of HCMV infection on atherosclerosis (AS) possible mechanisms. Methods PCR, HCMV infection in SMCs time points after the detection IE gene, L gene of the DNA, while antisense oligonucleotide and the role of ganciclovir in infected cells, the dynamic observation of its IE gene, L gene inhibition. Results infected cells could be detected 12 h IE gene, 24 h could be detected in both IE gene and L gene, detected only 48 and 72h L gene. the role of ganciclovir after IE gene and L gene is relatively weakened. The antisense oligonucleotide (ASON), after effect, IE gene was obviously inhibited, L gene was significantly reduced. Conclusion SMCs infected IE gene, L gene expression and regulation may be in the HCMV To the pathogenesis and treatment of AS has an important significance. [Keywords:] smooth muscle cell; cytomegalovirus; IE gene; L gene; atherosclerosis Human cytomegalovirus (HCMV) antigen and DNA sequences present in atherosclerosis (AS) in the pathological tissues were mainly observed in vascular smooth muscle cells (SMCs) and endothelial cells (EC) (1), show that HCMV infection may promote the AS the occurrence and development (2,3), but its pathogenic mechanism is unclear. HCMV genes known to be expressed in infected cells of a certain sequence, its sequence is divided into immediate early (IE), early (E) and late (L) of three, which, IE and L genes were detected in HCMV reflect the transcription, translation, replication of the different biological stages of their interaction with the biological characteristics of infected cells, therefore, this study, after HCMV infection of SMCs IE and L gene expression and regulation