Human endometriosis eutopic and ectopic endometrial cells in primary culture and morphological observation.docVIP

 PAGE \* MERGEFORMAT 22 Human endometriosis eutopic and ectopic endometrial cells in primary culture and morphological observation Author: Chao-Qin Yu, Shu-Fang Shi, Yu-Huan Liu, H. Wang, Yan-Hua Song, Yu Jin [Keywords:] Endometriosis [Abstract] Objective: To investigate the endometriosis (endometriosis, EM) ectopic endometrial cells in primary culture method, and compare the EM eutopic and ectopic endometrial cells and normal eutopic endometrial cell morphology differences. Methods: The modified EM method of primary culture cells, immune cells, cell types identified by chemical method, in the optical microscope and electronic microscope observation of cell morphology. Results: The normal endometrial cells and EM in eutopic and ectopic endometrial cells were isolated and cultured the success rate of 91.67%, 93.75% and 75.00%. EM ectopic, eutopic endometrial glandular epithelial cells with normal eutopic endometrial glandular epithelial cells, similar in size, but the EM ectopic endometrial glandular epithelial cells increased chromatin, the nuclear increase. EM ectopic endometrial stromal cells compared with EM and normal eutopic eutopic endometrial stromal cells is small, and have more cell surface microvilli and cytoplasmic processes. Conclusion: The attention drawn method, using modified method of primary culture can improve the EM ectopic endometrial cell culture success rate. EM ectopic endometrial glandular epithelial cells and stromal cells with normal ultrastructure of women and EM eutopic endometrial cells was markedly different. [Keywords:] Endometriosis; primary culture; glandular epithelial cells; mesenchymal cells Primary culture and morphologic observation of eutopic and ectopic endometrial cells from patients with endomehiosis ABSTRACT Objective: To explore the method of primary culture for endometriotic cells and to find out the differences in morphological manifestations among endometriotic cells and eutopic endometrial cells sampled from