Human epidermal stem cell culture technology.docVIP

 PAGE \* MERGEFORMAT 7 Human epidermal stem cell culture technology Of: Wang Xiuqing Wang Zhiling Liu Huimin [Keywords:] epidermal stem cell; cell culture; cell passage In recent years, human tissue stem cell research has become the focus of scholars, epidermal stem cells is one of them. Epidermal stem cells are maintained in adulthood but also higher self-renewal capacity of cells in the normal state to maintain self-renewal of the epidermis, when stimulated by injury, the epidermal stem cells can participate in the repair of skin damage [1]. With the speed of population aging, chronic ulcers and skin diseases increased, coupled with burns and skin defects caused by accidental injury clinical to the growing demand for skin transplantation. As a small number of epidermal stem cells, the lack of clear specific molecular markers, in vitro stem cells easily lose their biological characteristics, increasing the difficulty of isolation and culture. to obtain a large number of highly purified epidermal stem cell research as the key technology in this field. We in the cell culture according to terms of years of work experience, the human epidermal stem cells in culture are summarized below. A human epidermal stem cells isolation and culture Application of rapid adhesion to collagen type IV method from surgical resection of isolated human foreskin epidermal cells in the rapid adhesion of cells, this group of cells is considered to be stem cells, it has a rapid adhesion, slow growth characteristics [2]. Take ages 5 to 25 years of age in patients without urinary tract infection Pippi film package, under sterile conditions to remove the subcutaneous fat layer, containing penicillin, streptomycin, PBS wash skin graft repeated several times, cutting into a size of about 0.5 cm 0.5 cm the skin graft, placed in a Petri dish, added 0.25% protease 10 ml, 4 digestion l4 ~ 16 h, suction supernatant was separated epidermis and dermis. cut and epidermis, with 0.25%