HPLC-ELSD method for the determination Qingshenjianfei the content of the film Astragaloside_0.docVIP

 PAGE \* MERGEFORMAT 9 HPLC-ELSD method for the determination Qingshenjianfei the content of the film Astragaloside Study: LI Zhan, Limei Rong ON TRADITIONAL Nguyen Group [Abstract] Objective To establish the HPLC-ELSD determination Qingshenjianfei Astragaloside film content. Methods used C18 column (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile - water (35:65). Evaporative temperature 120 ℃, atomization temperature of 80 ℃, carrier gas flow rate 1.6 mL / min, column temperature of 35 ℃. Results astragaloside range of 1.03 ~ 8.24 μg showed a good linear relationship, r = 0.999 9, the average sample recovery rate of 99.35%, RSD = 1.36% (n = 6). Conclusion The method is simple, accurate, reproducible, high precision, can be used to Qingshenjianfei the quality control. [Keywords:] Qingshenjianfei film astragaloside, high performance liquid chromatography - evaporative light scattering detection, determination Abstract: Objective To establish a HPLC-ELSD method for determining the content of astragaloside Ⅳ in Qingshen Jianfei Tablets. Methods Stationary phase was C18 column (4.6 mm × 250 mm, 5 μm), mobile phase was acetonitrice-water (35:65) . The evaporation temperature was 120 ℃, the pulverization temperature was 80 ℃, the flow rate was 1.6 mL / min and column temperature was 35 ℃. Results The standard curve was linear within the range of 1.03 ~ 8.24 μg, r = 0.999 9. The average recovery was 99.35% with RSD = 1.36% (n = 6). Conclusion The established method is simple and accurate, with good reproducibility and high precision, and suitable for the determination of astragaloside Ⅳ in Qingshen Jianfei Tablets. Keywords:: Qingshen Jianfei Tablets, astragaloside Ⅳ, HPLC-ELSD, content determination Qingshenjianfei film is rhubarb, hawthorn, Alisma, Astragalus, Salvia, etc. have been made against the traditional Chinese medicine, with a light body weight, spleen qi, blood circulation, Chest cured product of the effect, patients are for obesit