HPLCELSD determination of glycyrrhizic glycyrrhizin in contents 3.docVIP

 PAGE \* MERGEFORMAT 11 HPLCELSD determination of glycyrrhizic glycyrrhizin in contents 3 Study: U.S. YE Xiao Tian, soup should worship, Huang Yayan [Abstract] Objective To determine the glycyrrhizin in Radix Glycyrrhizae G2, ammonium licorice, Glycyrrhiza uralensis saponins B content. Methods using HPLC ELSD method, Hypersil C18 column (5 m, 4.6 mm 150 mm), methanol and ammonium acetate solution (0.2 mol / L) and acetic acid (volume ratio 65:34:1) as mobile phase, flow rate 1.0 mL / min, ELSD drift tube temperature of 110 , carrier gas (N2) flow rate of 2.8 L / min. Results glycyrrhizin G2, ammonium licorice, Glycyrrhiza uralensis saponins B of the linear range of 6.02 ~ 120.4,13.22 ~ 264.4,6.32 ~ 126.4 g / mL (r values were 0.999 6,0.999 7,0.999 8), the average recovery (n = 6 ) was 100.6%, 100.8% and 99.87%. Conclusion The method is simple, reproducible, and can be used for licorice glycyrrhizin G2, licorice ammonium uralensis saponins B content. [Keywords:] licorice; glycyrrhizin G2; licorice ammonium; uralensis saponins B; HPLC ELSD Abstract: Objective o establish a quantitative method for determining the content of saponin G2, ammonium glycyrrhizinate and uralsaponin B in Glycyrrhiza uralensis. Method HPLC ELSD method was used to analyze the three constituents. Samples were separated on an Hypersil C18 column (5 m, 4.6 mm 150 mm) by using methanol 0.2 mol L-1 ammonium acetate glacial acetic acid (65:34:1) as mobile phase. The flow rate was 1.0 mL min-1 and the temperature of drift tube was set at 110 . The gas flow (N2) was set at 2.8 L min-1. Results The linear ranges of saponin G2, ammonium glycyrrhizinate and uralsaponin B were 6.02-120.4, 13.22-264.4 and 6.32 ~ 126.4 g mL-1, respectively and their average recoveries (n = 6) were 100.6%, 100.8% and 99.87%, respectively. Conclusion The method is convenient, reliable and can be used for analyzing saponin G2, ammonium glycyrrhizinate and uralsaponin B in Glycyrrhiza uralensis. Keyword