HPLCELSD determination of 3 kinds of Platycodon grandiflorum saponins.docVIP

 PAGE \* MERGEFORMAT 10 HPLCELSD determination of 3 kinds of Platycodon grandiflorum saponins Of: Ye Jing, the United States Tim Shaw, Tom have to worship, Huang Yayan [Abstract] Objective To determine the Platycodon Radix Platycodin D, D3, E content. Methods using high performance liquid chromatography with evaporative light laser Shanshe detector (HPLC ELSD) method, Hypersil C18 column (5m, .6 mm 150mm), acetonitrile - water as mobile phase gradient elution 1.0mL/min, ELSD drift tube temperature of 113 , carrier gas (N2) flow rate 3.0L/min. Results platycodin D, D3, E the linear range of 13.78 ~ 275.6g/mL (r = 0.9995), 8.40 ~ 168.0g/mL (r = 0.9997), 12.02 ~ 240.4g/mL (r = 0.9996), the average recovery (n = 5) was 98.3%, 99.4% and 101.3%. Conclusion The method is simple, accurate and reliable and can be used platycodin D, D3, E content. [Keywords:] Campanulaceae; platycodin; HPLC ELSD ABSTRACT: Objective To establish a quantitative method to determine the content of platycodin D, D3 and E in Radix platycodi.Methods High performance liquid chromatagraphy evaprorative light scattering dector (HPLC ELSD) method was adopted. Platycodin D, D3 and E were separated by C18 (5m, 4.6mm 150mm) with acetonitrile water as mobile phase in a gradient program; flow rate was 1.0mL/min, the temperature of drift tube was set at 113 , and the gas flow (N2) was set at 3.0L / min.Results The linear ranges of platycodin D, D3 and E were 13.78-275.6g/mL (r = 0.9995), 8.40-168.0g/mL (r = 0.9997) and 12.02-240.4g/mL (r = 0.9996) , respectively. The average recoveries (n = 5) were 98.3%, 99.4% and 101.3%, respectively.Conclusion The method is convenient, accurate and reliable, and can be used for the determination of platycodin D, D3 and E in Radix platycodoi . KEY WORDS: Radix platycodoi; platycodin; HPLC ELSD Chinese bellflower (Radix platycodi) plant for the Campanulaceae Campanulaceae Platycodon grandiflorum (Jacq.) A.DC. the dried root, in the long history of me