HPLCDADELSD method for simultaneous determination of oleanolic acid in Radix Clematis and ivy sapogenin content.docVIP

 PAGE \* MERGEFORMAT 10 HPLCDADELSD method for simultaneous determination of oleanolic acid in Radix Clematis and ivy sapogenin content [Abstract] Objective To establish the evaporative light scattering detector (ELSD) and diode array detector (DAD) Determination of oleanolic acid in Radix Clematis and ivy sapogenin high performance liquid chromatography. Methods Column: Merck Lichrocart C18 column (4.0 mm 250 mm, 5 m); mobile phase: methanol water glacial acetic acid triethylamine (volume ratio 87:13:0.04:0.02); flow rate: 1.0 mL min-1; column temperature: 30 ; DAD detection wavelength was 210 nm; ELSD evaporation tube temperature is 80 , the gas pressure of 172.375 kPa. Results ivy sapogenin oleanolic acid linear range of 1.43 ~ 14.3 g, and 2.1 ~ 21.0 g; Ivy saponins Yuan and oleanolic acid in order to increase detection DAD recoveries were 98.02%, 98.94%, RSD was 2.61%, 2.34%; in the ELSD detection of the recoveries were 98.72%, 100.03% , RSD 2.51%, respectively, 2.22%. Conclusion The method is simple, reproducible, specific, and to improve quality control standards Clematis herbs provide a scientific basis. [Keywords:] Clematis; oleanolic acid; ivy sapogenin; performance liquid chromatography; evaporative light scattering detector; diode array detector Abstract: Objective To determine the content of oleanolic acid and hederagenin from Radix Rhizoma Clematidis by RP HPLC ELSD DAD at the same time. Methods A Merck Lichrocart C18 column (4.0 mm 250 mm, 5 m) was used. The mobile phase was consisted of methanol water acetic acid triethyl amine (87:13:0.04:0.02) with a flow rate of 1.0 mL min-1, and the column temperature was 30 . The DAD detection wavelength was 210 nm; The ELSD drift tube temperature was 80 , and gas pressure was 172.375 kPa.Results The linear ranges of hederagenin and oleanolic acid were 1.43-14.3 g and 2.1-21.0 g respectively. The average recovery of hederagenin and oleanolic acid were 98.02% (RSD = 2.61%), 98.94 % (RSD = 2.34%)