HPLC-MS-MS determination in rabbit plasma levels Astragaloside.docVIP

 PAGE \* MERGEFORMAT 15 HPLC-MS/MS determination in rabbit plasma levels Astragaloside [Abstract] Objective To establish a rabbit plasma containing drug Astragaloside new method for rapid determination of content. Method for solid phase extraction for enrichment in plasma astragaloside; Agilent SB-C18 reverse phase chromatography to separate; multi-stage ion trap mass spectrometry response mode to m / z 807.5 627.1 for the selected ion pair content of the internal standard method. Results Astragaloside plasma detection limit of 0.1ng/mL, the linear range of 0.5 ~ 50.0g/mL, the average method recovery rate of 96.3% ~ 104.7% range. Conclusion This method has high sensitivity, good stability and fast, in line with the analysis of biological samples request, expected to be used Astragaloside pharmacokinetics. [Keywords:] astragalus; astragaloside; solid phase extraction; HPLC-MS/MS ABSTRACT: ObjectiveTo establish a new method for the determination of astragaloside in rabbit plasma by high performance liquid chromatography-ion trap mass spectrometry (HPLC-MS/MS). MethodsAstragaloside in plasma samples was enriched by solid phase extraction. The resulted samples were further separated by reverse phase chromatography with Agilent SB-C18 column. Multiple reaction mode of MS / MS was employed to determine the concentration of astragaloside by internal standard with m / z 807.5 627.1 as the selected ion pair.ResultsThe detection limit of astragaloside in plasma samples was 0.1ng/mL, the linear range was 0.5-50.0g/mL, and the average recovery rate of the method was within the range of 96.3% -104.7%. ConclusionThe proposed method has the characteristics of high sensitivity, good stability and high speed. These properties can meet the requirements of the analytical method for biological samples, which indicates that the method may be used in pharmacokinetic study of astragaloside . KEYWORDS: Radix Astragali; astragaloside ; solid phase extraction; high perfor