HPLCMS Determination of different areas of freeze-dried fresh Houttuynia content of flavonoids.docVIP

 PAGE \* MERGEFORMAT 12 HPLCMS Determination of different areas of freeze-dried fresh Houttuynia content of flavonoids Authors: Meng-jiang, Liao Wei, Dong Xiaoping, Zhao Zhong-zhen [Abstract] Objective To investigate the different areas of freeze-dried fresh Houttuynia flavonoid content of active ingredient. Methods HPLC  MS method, Alltima C18 column; mobile phase: volume fraction of 0.2% acetic acid solution as mobile phase A, acetonitrile as mobile phase B, gradient elution: 0 ~ 20 min, 5% ~ 20% B; 20 ~ 40 min, 20% ~ 20.5% B; 40 ~ 50 min, 20.5% ~ 35% B; flow rate: 0.6 mL / min; post and triage: 0.4 mL / min into the mass spectrometer; UV and MS detection at the same time. The results of the Hong Kong production of flavonoids in Houttuynia 5 concentrations are lower than the Emei, Sichuan and Guangdong origin, and Sichuan Emei origin rutin, hyperin, quercitrin levels were higher than those produced in Guangdong, and Guangdong origin of the oak Pi Su  3  O  β  D  galactose  7  O  β  D  glucoside and quercetin  3  O  α  L  rhamnose  7  O  β  D  glucose glycosides were higher than those in Sichuan Emei origin. Conclusions different areas of freeze-dried fresh Houttuynia flavonoid content of different active ingredients. [Keywords:] freeze-drying of fresh Houttuynia; HPLC  MS; flavonoids; Determination Quantitative analysis of flavonoids in freeze  dried Houttuynia cordata from different habitats by HPLC  MS Abstract: Objective To compare the contents of flavonoids in freeze  dried Houttuynia cordata from different habitats. Methods Samples were analyzed by HPLC  MS using an Alltima C18 column. The mobile phase consisted of 0.2% acetic acid in water (A) and acetonitrile ( B) using a gradient elution program of 20% (B) in 0 ~ 20 min, 20% ~ 20.5% (B) in 20 ~ 40 min, 20.5% ~ 35% (B) in 40 ~ 50 min. The flow rate was 0.6 mL / min. Results The contents of five flavonoids in HongKong were higher than those in