HPLCMS method for determination of plasma concentrations of carbocisteine.docVIP

 PAGE \* MERGEFORMAT 10 HPLCMS method for determination of plasma concentrations of carbocisteine Of: Li Chan, Fu Qiang, Yang Jianting, Li Feng Wu [Abstract] Objective To establish a sensitive, efficient and fast performance liquid chromatography mass spectrometry method for the determination in human plasma carbocisteine blood concentration. Methods 100mL / L perchloric acid precipitation of plasma proteins, the supernatant RP high performance liquid chromatography, electrospray ion trap mass spectrometry in plasma samples carbocisteine, multi-level response mode selected ion quantification. Results The detection limit is 0.01g/mL, the linear range of 0.2 ~ 200.0g / mL, recoveries of 89.2% to 105.6%. Conclusion The method is precise, sensitive, accurate, and can be used for biological samples in the determination of carbocisteine. [Keywords:] carbocisteine; high performance liquid chromatography-ion trap mass spectrometry; plasma concentration ABSTRACT: Objective To establish a sensitive, valid and rapid high performance liquid chromatography trap mass spectrometry (HPLC MS) method for determining carbocisteine concentration in human plasma. Methods Plasma samples were pretreated by 100mL / L perchloric acid to remove the interference protein. The supernatant was separated by reverse phase HPLC, electrospray MS was used to detect carbocisteine in plasma samples, and selective ion method of multiple reaction modes was used to determine the concentration of carbocisteine. Results The limit of detection was 0.01g/mL and the linear range was 0.2-200.0g/mL. The recovery rate was within the range of 89.2% -105.6%. Conclusion The proposed method could be used to determine carbocisteine in biological samples. KEY WORDS: carbocisteine; high performance liquid chromatography trap mass spectrometry; plasma drug concentration U.S. carboxylase pseudoephedrine tablets are widely used clinically as a cold medicine, the main components of carbocisteine, pseu