HPLCELSD determination of βlactam antibiotic injection in the content of arginine.docVIP

 PAGE \* MERGEFORMAT 18 HPLCELSD determination of βlactam antibiotic injection in the content of arginine [Abstract] Objective To establish a high-performance liquid chromatography  evaporative light scattering detection (HPLC  ELSD) analysis of β  lactam antibiotics with the preparation of L-arginine content. Methods Agilent Zorbax SB  C18 (4.6mm * 250mm, 5μ m) column, mobile phase 0.1% trifluoroacetic acid solution: acetonitrile (80:20), flow rate 0.8ml/min, drift tube temperature of 45 ℃ , fog of gas pressure to 3.5bar. The results for the test products in the Arginine with antibiotic drugs and related impurities in the primary separation is good, within the framework of 10.27 ~ 314μ g/ml was a good linear relationship (r = 0.99986), in the cefepime hydrochloride in the average recovery rate to 101.5%, RSD of 2.1% (n = 9); in the aztreonam Yugoslavia and the average recovery was 97.8%, RSD of 1.8% (n = 9); in the cephradine in the average recovery was 95.6%, RSD of 1.9% (n = 9). To Cefepime Hydrochloride for Injection as an example, repetitive tests, the results measured with L-arginine mean 37.0%, RSD of 1.9% (n = 9), the sensitivity test measured, the detection limit and limit of quantification were 2 and 10μ g/ml. Conclusion The method is proprietary, fast, accurate and reproducible, can be used as part of the β  lactam antibiotics with the preparation of active substances added method for determination of arginine. [Keywords:] β  lactam antibiotics; arginine; determination; high-performance liquid chromatography; Evaporative Light Scattering Detector ABSTRACT Objective To develop an HPLC  ELSD method for determination of arginine in β  lactam antibilotic injection. Methods The column: Agilent Zorbax SB  C18 (4.6mm * 250mm, 5μ m); the mobile phase: 0.1mol / L trifluoroacetic acid  methanol (80:20); the drift tube temperature: 45 ℃ ; the pressure of nebulizing gas: 3.5bar. Results Good separation of arginine and main related subs