HPLC-ELSD method for the determination on deaf Zuo Ci Pill ursolic acid.docVIP

 PAGE \* MERGEFORMAT 7 HPLC-ELSD method for the determination on deaf Zuo Ci Pill ursolic acid [Abstract] Objective To establish a high performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) Determination of deafness Zuo Ci Pill ursolic acid. Method column VP-ODS C18, using methanol -0.5% acetic acid (90:10) as mobile phase, flow rate: 0.8 mL / min. Results ursolic acid in the 63 ~ 630 μg / mL was linear within the range (r = 0.999 9), the average recovery was 98.11% (RSD = 1.14%). Conclusion The method is simple, accurate, reproducible, and can be used for the preparation of the content. Keywords:] deaf Zuo Ci Pill, ursolic acid, high performance liquid chromatography - evaporative light scattering detector Abstract: Objective The method for determination of ursolic acid in Erlongzuoci pills by HPLC-ELSD was established. Methods VP-ODS C18 column was used with the mobile phase of methanol-0.5% acetic acid (90:10). The flow rate was 0.8 mL / min. Results Ursolic acid showed a good linear relationship at the range of 63 ~ 630 μg / mL (r = 0.999 9). The average recovery was 98.11% (RSD = 1.14%). Conclusions The method is simple, accurate and reproducible. It can be used for determination of ursolic acid in Erlongzuoci pills. Keywords:: Erlongzuoci pills, Ursolic acid, HPLC-ELSD Zuo Ci Pill deaf from the 2005 edition of the original standard lt;lt;Chinese Pharmacopoeiagt; “(a), by the magnet, Rehmannia glutinosa, Cornus officinalis, Paeonia suffruticosa, yam etc., with a nourishing role Pinggan for liver kidney, tinnitus and deafness, dizziness treatment. the original standard [1] determined by TLC scanning the content of ursolic acid from Cornus officinalis, but there is not easy to operate TLC, influenced by the experimental conditions, inadequate and poor reproducibility. We build high performance liquid chromatography - evaporative light scattering detection (HPLC-ELSD) Determination of ursolic acid preparations,