HPV 16L1 prokaryotic expression plasmid.docVIP

 PAGE \* MERGEFORMAT 7 HPV 16L1 prokaryotic expression plasmid Study: Left Feng Qiong Zhao Sulan Liwan Yi Ouyang Xiaohong Lv Mei Yun Wei Li Jiang Zhonghua Li Mingyuan [Abstract] Objective: To construct a prokaryotic expression of HPV 16L1 plasmid, for the next step of protein expression and function of proteins in preparation. Methods: The clinical HPV16 positive samples as templates, PCR amplification of fragments of L1, L1 fragment EcoR Ⅰ and Sal Ⅰ by double digestion, inserted into the vector pGEX4T 1, into JM109 competent cells, flat screened positive plasmid pGEX4T 1/HPV L1. by restriction enzyme digestion, plasmid sequencing accuracy. Results: The prokaryotic expression plasmid pGEX4T 1/HPV16L1, and through digestion, sequencing and other methods to validate correct. Conclusion: The successful construction of the pGEX4T 1/HPVL1 for future protein expression and functional studies to lay a solid foundation for the HPV16 vaccine study provides useful support. [Keywords:] Human papillomavirus type 16, plasmid construct Cervical cancer is one of common malignant tumors in women worldwide each year about 370,000 new cases, 20 million women die of cervical cancer. In recent years, HPV (human papillomaviruses, HPV infection is considered to most cervical closely related to the occurrence and development of cancer [1]. It is reported that more than 99% of the cervical part of the tumor could be detected the presence of HPV DNA. Therefore, the study HPV vaccine for cervical cancer prevention and treatment has an important role. papillomas have been found so far Up to 100-type virus, which is most closely related HPV16 and cervical cancer. HPV oncoprotein E5, E6, E7 can stimulate the growth and transformation function [2,3]. E5 is a membrane or membrane integration of proteins. Early in the infection of cell clones breeding, play an important role in the expansion. HPV L1 coat protein is protective antigen, with a strong conservative: in the choice of p