HPLCMS method for the determination in rabbit plasma and tissue levels of isopropyl danshensu.docVIP

 PAGE \* MERGEFORMAT 14 HPLCMS method for the determination in rabbit plasma and tissue levels of isopropyl danshensu Of: Zhang Qun is, Lizhong Jin, Wang Shixiang, South Ye Fei, Yu Jie, Zheng Xiaohui [Abstract] Objective To establish a rabbit plasma and tissues of (3,4 dihydroxyphenyl) isopropyl @-hydroxy acid (Danshensu isopropyl) high performance liquid chromatography electrospray ion trap mass spectrometry (LC ESI MSn) New measurement methods. Methods A liquid extraction method of plasma and tissue samples to 4 methylparaben as the internal standard, LC ESI MSn mode multi-level response to plasma and tissue levels of isopropyl Danshensu. Results Danshensu isopropyl in plasma, heart, liver, spleen, lung, kidney and brain of the detection limits were: 0.54,1.08,1.20,1.62,2.15,1.12,1.36 ng / mL, linear range: 0.20 ~ ~ ~ 200,0.01 400,0.10 400,0.20 100,0.02 ~ ~ 100 ~ 800,0.01 and 0.05 ~ 100g/mL, day precision relative standard deviations were less than 10%, and the average recovery of 89.5 % ~ 107.4% range. Conclusions The analysis method is sensitive, specific, for the isopropyl Danshensu non-clinical and clinical pharmacokinetic study provides a methodology for reference. [Keywords:] Danshensu isopropyl, HPLC MS, pharmacokinetics ABSTRACT: Objective To establish a new method for the determination of isopropyl 3 (3,4 dihydroxyphenyl) 2 hydroxypropanoate (IDHP) in plasma and tissue in rabbits by high performance liquid chromatography electrospray ion trap mass (HPLC ESI MSn). Methods Plasma and tissue samples were pretreated by liquid liquid method, methyl 4 hydroxybenzoate was used as the internal standard, and the multi reaction mode was applied to determine the concentration of IDHP in plasma and tissue samples. Results The detecting limits of IDHP in plasma, heart, liver, spleen, lung, kidney and brain were 0.54, 1.08, 1.20, 1.62, 2.15, 1.12 and 1.36ng/mL, respectively; the linear ranges were 0.20-400.0, 0.10-200.0, 0.01-100.0, 0.02-400.0, 0.20-8