HPLC-ELSD determination of tonic tablets Tribulus terrestris content of astragaloside.docVIP

 PAGE \* MERGEFORMAT 9 HPLC-ELSD determination of tonic tablets Tribulus terrestris content of astragaloside Authors: YANG Qing-sheng, Li Cai-Rong, Zhao-hui Abstract: Objective: To establish a tonic tablets Tribulus terrestris astragaloside method for the determination. Methods: HPLC - evaporative light scattering detection (HPLC-ELSD), right tonic tablets Tribulus terrestris astragaloside to assay. Column was ODS-C 18 column (250mm * 4.6mm, 20μ m); mobile phase: acetonitrile - water (1:2); injection volume 10μ L; room temperature; flow rate 1.0mL/min; ELSD parameters: drift tube temperature of 100 ℃ , gas flow rate 2.74mL/min. Results: astragaloside reference substance in the range of 2.024 ~ 10.120μ g within the sample volume between the peak area and showed a good linear relationship, r = 0.9996, average recovery was 99.26%. Conclusion: The method is simple, accurate, reproducible, and can be used to control the quality of the preparation. Keywords:: Tribulus terrestris tonic tablets; Astragaloside; HPLC - evaporative light scattering detection method; Determination Department of ministerial standard tonic tonic tablets Tribulus terrestris Tribulus terrestris pill to change varieties, from Astragalus (Mizhi), Atractylodes (bran fried), Chinese yam, Poria, white lentils, Gorgon (bran fried), Angelica, Astragalus complanatus, dodder seed, dried tangerine peel, etc. 10 Chinese herbal medicines by modern processing technology made film-coated tablets, with spleen kidney, Yiqimingmu the function for the spleen and lack of eyes dim, depending on the material is unclear, lumbar acid, shortness of breath embolism 〔 1〕 . Astragalus is the prescription of the monarch herb, therefore, is a tonic astragaloside Tribulus terrestris is one piece of the active ingredient. In order to control the quality of tonic tablets Tribulus terrestris to ensure its efficacy, we used high-performance liquid - evaporative light scattering detection (HPLC-ELSD) was determined by ast