HPV detection and typing of genes Progress.docVIP

 PAGE \* MERGEFORMAT 19 HPV detection and typing of genes Progress [Abstract] epidemiology and molecular biology data show that human papillomavirus (HPV) infection can cause uterine epithelial neoplasia (CIN) and cervical cancer. And HPV disease unique power of the different types there are also differences high-risk type, the sustained high viral load infection is an important factor to promote cervical cancer. Thus, HPV infection in the early detection, accurate classification and quantification of the virus to cervical cancer is important. of the gene of HPV detection and typing methods such as nucleic acid hybridization, PCR, quantitative PCR, as well as the advantages and disadvantages of each method are reviewed. [Keywords:] Human papillomavirus; polymerase with reaction; real-time fluorescence quantitative; molecular beacons; Literature Review HPV (human papillomavius, HPV) is to promote uterine epithelial neoplasia (cervical intraepithelial neoplasia, CIN) and cervical cancer an important factor known [1-2]. German scholar Harald zur Hausen for his discovery of the HPV and the relationship between cervical cancer won the 2008 Nobel Prize in Physiology or Medicine. HPV with a variety of types, divided into high risk and low risk, cancer-causing potential of different types there are differences. high-risk, high viral load persistent infection can cause cervical cancer [3] In 2009, held May 25th International Papillomavirus Conference, indicating that HPV infection rate of the global upward trend, and the emergence of new genotypes [4] . In China, the incidence of cervical cancer are also increasing and tends to younger, therefore, HPV detection of infection, viral quantification, genotyping, cervical cancer prevention and treatment of clinical significance. on the basis of the relevant data, we gene of HPV detection and typing methods such as nucleic acid hybridization, PCR, quantitative PCR, as well as the advantages and disadvantages of each