HPLC-DAD Determination of turmeric in the Northwest of Hubei Province diosgenin content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC-DAD Determination of turmeric in the Northwest of Hubei Province diosgenin content [Keywords:] Turmeric; diosgenin; Determination Turmeric for our unique varieties of Dioscoreaceae Dioscorea Dioscorea Dioscorea yam (Dioscorea Zingiberensis CHWright) of the rhizomes. Their sexual sweet, bitter cold, with Qingfei cough, Lishi Tonglin, Tongluo pain, swelling detoxification function. The main ingredients of turmeric steroidal saponin, diosgenin roots in the composition of the myocardial ischemia, Chest Pain, hyperlipidemia and so has obvious therapeutic effect [1]. China’s cultivated area of tens of thousands of hectares of turmeric, including Hubei, northwestern Hubei Region 2 million hectares, is the turmeric planted earlier and more concentrated areas. At present, many using the weight method, TLC, HPLC-ELSD method study or preparation of such medicines in total saponin or sapogenin content of this paper, HPLC-DAD method ginger herbs in the diosgenin content of the measurement results show that The method is accurate and reliable, reproducible, in order to better control the intrinsic quality of turmeric herbs provide a reliable analysis method. An instrument and reagent Germany Dionex-U3000 liquid chromatography (diode array detector, Dionex Chemeleon chromatography workstation), diosgenin reference substance (for determination by the Chinese Pharmaceutical and Biological Products Institution, batch number 111707-200501), methanol (Tianjin Tai Mao chromatography pure), D-101 macroporous resin (Tianjin Sea Light Chemical Co., Ltd.); water as a double-distilled water, other reagents were analytical pure. 5 copies of turmeric herbs Yunxi from Hubei Province, Yun County, Shennongjia, Chushan, the Housing County, through the School of Pharmacy, Hubei College of Traditional Chinese Medicine Teaching and Research ZHAN Ya-hua, Professor identification identified as Dioscoreaceae Dioscorea of the rhizome of Dioscorea. 2 Methods and Resu