HPLC-MS Determination of roxithromycin in human plasma concentration of.docVIP

 PAGE \* MERGEFORMAT 7 HPLC-MS Determination of roxithromycin in human plasma concentration of [Abstract] Objective To develop a liquid chromatography - mass spectrometry in plasma concentration of roxithromycin approach. Method column was Thermo Hypersil-HyPURITY C18 (150 * 2.1nm, 5μ m); mobile phase of 10mM ammonium acetate (1.5% acetic acid, 0.1% TFA): methanol: acetonitrile (38:365:26); electrospray ionization source (ESI); selective positive ion monitoring (SIM), mass charge ratio (m / z) of roxithromycin 838, 815 Midecamycin. The results of blood drug concentration of roxithromycin in 25 ~ 16000ng/ml a good linear relationship between the low, middle and third year, the average concentration of the relative recoveries were 98.6%, 100.2% and 99.4%, days between the relative RSD are less than 15%. Conclusion The method is simple, rapid, accurate, high sensitivity, can be used for the treatment of roxithromycin drug monitoring and pharmacokinetics studies. [Keywords:] roxithromycin; high performance liquid chromatography - mass spectrometry; blood drug concentration Roxithromycin (roxithromycin) derived from erythromycin, erythromycin antibacterial spectrum and the same, but stronger antibacterial activity than erythromycin, especially Haemophilus influenzae, Neisseria gonorrhoeae bacteria, Escherichia coli, Bacillus pertussis strong antibacterial activity against Chlamydia pneumoniae, Mycoplasma pneumoniae, Legionella bacteria cells the role of pathogenic micro-organisms than erythromycin. Which is characterized by pairs of gastric acid stability, oral absorption is good, the organization penetrating strong peak plasma concentration of high, widely distributed, and less adverse reactions [1]. Roxithromycin on the blood drug concentration measurement methods, reported in the literature most likely to be microbiological method [2 ~ 4], operating complexity and the large systematic errors affecting the accuracy of the results; also been detected using H