Human DNA methyltransferase 3B1 eukaryotic expression vector and identification of.docVIP

 PAGE \* MERGEFORMAT 10 Human DNA methyltransferase 3B1 eukaryotic expression vector and identification of Of: the tree of the original Shao Jiang Lihua Gongxiang Feng Zhou Zhang Sheng States Agency [Abstract] Objective: To construct the DNA methyltransferase 3B1 (DNMT3B1) eukaryotic expression vector pCMV-DNMT3B1. Methods: According to the Genebank sequence of the human DNMT3B1 cDNA specific primers were designed and synthesized, HCT116 cells extracted total RNA, using PCR technology amplified DNMT 3B1, and amplified product was cloned into the eukaryotic expression vector pCMV-2B in. The results: PCR people DNMT3B1 of the cDNA was amplified fragment size is 2.6 kb, recombinants using restriction enzyme digestion PCR identification and DNA sequence analysis of eukaryotic expression vectors by pCMV-DNMT3B1. Conclusion: The successfully constructed the eukaryotic expression vector DNMT3B1 for further research DNMT3B1 provided the conditions. [Keywords:] DNA methyltransferase 3B1; construction; identification Abstract Objective: To construct and identify the eukaryotic expression plasmid for human pCMV-2B-DNMT 3B1. Methods: Accord to the published human cDNA sequence in Genebank, a pair of primers were respectively designed and synthesized. The total RNA was isolated from HCT116 cell . After amplification with reverse transcription polymerase chain reaction (RT - PCR), the product was cloned into pCMV-2B vector. The recombinants were finally sequenced and identified by restrictive endonuclease digestion. Results: pCMV-DNMT3B1 eukaryotic expression vectors was successfully constructed, and it was identified by PCR, double restrictive endonuclease digestion and sequence analysis. Conclusion: The DNMT 3B1 eukaryotic expression vectors was successfully constructed and identified. Keywords: DNMT 3B1; Construction; Identification DNA methylation is a modification of eukaryotic genomes is an important way, DNA methylation occurs in CpG dinucleotide of