Human esophageal squamous cell carcinoma cells in vitro and biological properties.docVIP

 PAGE \* MERGEFORMAT 11 Human esophageal squamous cell carcinoma cells in vitro and biological properties Study: How to Train Xu Yu, Sun Zhigang, Sheng-Dong Huang, Fang Chaoying, Lin Xing, Zeng Zhiyong [Abstract] Objective To study the human esophageal squamous cell carcinoma cells in vitro and biological properties. Methods from primary esophageal squamous cell carcinoma specimens were obtained, the application digestion culture method for continuous passage cultured tumor cells, on the morphology of cultured cells observation, immunohistochemical identification, MTT determined cell number, growth curve and calculation of cell doubling time, colony colony formation experiments to determine the rate of cell cycle distribution by flow cytometry. Results of the tumors in 30 patients, 6 were successfully cultured tumor cells in vitro, cultured cells consistent with squamous cell characteristics, cell growth of the plateau, the cell doubling time (33.12 +-6.10h, colony forming efficiency (15.73 + -4.46%, (77.57 + -6.57) % cells in cell proliferation in G0, G1 phase, a small number of cells in active proliferation. Conclusion of human esophageal squamous cell carcinoma successfully cultured in vitro, the proliferation of tumor cells is different from only a few cells active. [Keywords:] esophageal cancer, carcinoma, squamous cell, tumor cells, cultured, tumor stem cells Currently, leukemia, multiple myeloma and lung cancer, ovarian cancer, breast cancer, neuroblastoma and other tumors of the research suggests that the proliferation of tumor cells is different from most of the tumor cells have lost growth potential, only a small part of the cell unlimited proliferation and tumor formation in vivo and the ability of these cells are called cancer stem cells, the tumor tissue with self-renewal, differentiation and proliferation of tumor cells, although only a small fraction of tumor cells, but it is the origin of tumor , development and transfer of the sourc