HPLC paeoniflorin in Radix Paeoniae Alba.docVIP

 PAGE \* MERGEFORMAT 9 HPLC paeoniflorin in Radix Paeoniae Alba Authors: Li Yuefeng, Yang Liang Wu, Shen Fei, YAN Xing Branch, Li Tingli [Abstract] Objective using high performance liquid chromatography (HPLC) Determination of paeoniflorin in different medicinal peony content, to determine which peony with the highest paeoniflorin. Methods column: Hypersil C18 column (4.6 mm * 200 mm, 5μ m); mobile phase: acetonitrile -0.05% phosphoric acid water (eighty-six past two p.m.); Detection: UV 230 nm; flow rate: 1.0 ml / min; column temperature: 25 ℃ . Results 2 years old, in October a short harvest storage life Bozhou the highest content of paeoniflorin peony. Conclusion The method is simple, accurate and reliable, and repeatable. [Keywords:] peony paeoniflorin high-performance liquid chromatography mass Abstract: ObjectiveTo compare the quality of different Radix Raeoniae Alba with HPLC methods. MethodsThe separation was carried out on Hypersil C18 column (4.6 mm * 200 mm, 5μ m); the mobile phase was acetonitrile-H2O (acidified to 0.05% with phosphoric acid) (14:86); the flow-rate was 1.0mml/min; the column temperature was 25 ℃ . ResultsThere was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time harvested in October with short storage time.ConclusionThe method is simple, rapid with good reproducibility. Keywords:: Radix Paeoniae Alba; Paeontflorin; HPLC; Quality Peony peony is Paeonia lactiflora Pall RANUNCULACEAE dry root, the main component of paeoniflorin (Paeoniflorin), Paeonia lactone glycosides (Albiflorin), oxidation of paeoniflorin (Oxypaeoniflorin), benzoyl paeoniflorin (Benzoylpaeoniflorin), commonly known as peony total glycosides (total glucosides of paeony, TGP). Have anti-inflammatory, anti-virus, antispasmodic analgesic, hepatoprotective and other pharmacological activity. TGP herbs and various formulations are paeoniflorin for quality control standards. An instrument and reagent 1.1 Instrument High-performa