Human fetal lung mesenchymal stem cells in radiation pneumonitis on the repair of lung injury.docVIP

 PAGE \* MERGEFORMAT 13 Human fetal lung mesenchymal stem cells in radiation pneumonitis on the repair of lung injury [Abstract] Objective To study the human fetal lung mesenchymal stem cells (MSC) of radiation pneumonitis in lung tissue repair. Methods 3 to 5 months of aborted fetuses, according to human bone marrow MSC in vitro methods to obtain human embryonic adherent lung cells to observe their morphology, cell cycle and differentiation potential in vitro. through the development of radiation pneumonitis, alveolar epithelial cells, determined in vitro using cell culture technology MSC human embryonic lung repair capacity of radiation injury of the lung tissue, contrast detection experiments, and TGF 1 in the control group content. Results embryonic lung MSC were cultured in vitro can be spread over 17 generations, the morphology and growth characteristics are not changed significantly in the G0/G1 phase of the cell number, with the typical features of stem cells. The immunophenotype type and similar to bone marrow-derived MSC and to effectively control after radiation injury of alveolar epithelial growth. Conclusion MSC can repair human fetal lung alveolar epithelial cells to promote growth, and can on lung epithelial cells after radiation damage repair. [Keywords:] human embryonic lung; mesenchymal stem cells; radiation pneumonitis Radiation-induced lung injury is the most common and serious complication of thoracic radiotherapy, the high incidence of tumor radiation dose limits, so that the decline in cancer therapy, some severe radiation-induced lung injury in patients with significantly increased risk of death. Was actively searching for to prevention, reversal and treatment of radiation-induced lung injury and the side effects of treatment became a very important topic. people in many tissues and organs have been found and the separation of multi differentiation potential of the corresponding stem cells, but in the lung tissue confirmed and i