Human fertility preservation and restoration of Research.docVIP

 PAGE \* MERGEFORMAT 15 Human fertility preservation and restoration of Research Of: Zoubao Lin Yuan Water Bridge in the words of Chau Zhou Yu Sun Qiang Xu Lei Wang Bingsong [Keywords:] Restoration of fertility preservation Human in vitro fertilization 30 years ago, technology (IVF the success of the restoration of human fertility provides a biological model of treatment, but also created a new era of assisted reproductive technology. And in the process of assisted reproduction, gametes, embryos and the preservation of reproductive organs is an integral part. With the development of low temperature biology, human gametes and embryos have long-term preservation in vitro can not affect its development potential, but most of the cancer through chemotherapy and radiotherapy in patients preservation of fertility is still our concern to the majority of assisted reproductive workers. While the development and maturation of gametes in vitro culture system to study also in its infancy, but the male and female gametes or gonadal tissue of frozen storage technology is still evolving. The new the safety of assisted reproductive technology are also issues of concern. 1 male fertility preservation and restoration of 1.1 Cryopreservation of sperm cryopreservation of human semen has a good foundation for the development technology, and is routinely used in IVF for the fine. However, semen cryopreservation damage in most of the original sperm (nearly 50%, and reduced sperm activity, it requires more than the amount of fresh semen frozen - thawed semen can be successfully fertilized (Holt, 2000. Therefore, semen cryopreservation of sperm and decrease for those patients with poor semen quality and, it is not so good , although intracytoplasmic sperm injection (ICSI’s development has been largely alleviated this problem. A recent study showed that out from the epididymis and testis sperm by freezing - thawing after ICSI, obtained similar to the fertilization rate (n