Human fetal blood of AGM stromal cell gene expression profile analysis of.docVIP

 PAGE \* MERGEFORMAT 23 Human fetal blood of AGM stromal cell gene expression profile analysis of Author: Fu Jin-rong, CHEN Hui-Qin, Xu-Chao Zhang, Shao-liang, ZHOU Dun-hua, Huang Ke Abstract In order to filter the aorta - gonad - mesonephros (AGM) stromal cells in the differentially expressed genes to medical abortion in pregnant 30-35 days, the source of human embryonic AGM zone stromal cells “experimental side” to the source Since the consequence of an accident abortion pregnancy from April to May of the cystic induction of fetal liver tissue stromal cells for the “drive side” was established in the human AGM stromal cells differentially expressed genes in the subtractive hybridization library. Further using gene chip technology created by suppression subtractive hybridization library was screened, and pick the significant difference in the expression of 18 clones were sequenced and homology analysis. The results showed that: In the building of the AGM suppression subtractive hybridization library, after the identification of a specific PCR amplification and the amplified band with a molecular weight of 200-700 bp clone has 211, the positive rate as high as 76.4%. Through gene chip screening, selected ratio value is greater than 5 clones sequenced a total of 18 shows that in the sequencing of the 18 significant differences in the expression of positive clones, 4 were for unknown genes, 14 known genes, of which These known genes were involved in cell migration, differentiation, growth, cell cycle, signal transduction and angiogenesis and other cellular key processes of life. Most of these genes in embryonic development of the role of hematopoietic differentiation has not been reported. Conclusion AGM stromal cells in the screening of differentially expressed genes for further study on the differentiation of embryonic hematopoietic development of the molecular mechanism of regulation provides the necessary theoretical basis. Keywords: A